詳細說明

Lolina A/S

Address: Sindalsvej 30 8240 Risskov Danmark 

                    Email: Info@lolina.dk

Product Specification

Product Description

Stem-Prom? supplement kit 2 is a sterile products which is Suitable for poorly attached or slow-proliferating primary or thawed BM-MSCs. Optimized for enhancing initial attachment and early expansion in adherent culture.

It has the following benefit:

Enhances Early Cell Recovery & Expansion: SafeGluta? ensures stable glutamine supply, promoting cell metabolism and recovery after thawing or seeding.

Boosts Stemness and Proliferation Signals: Higher Prolifex? Protein concentration provides robust support for stemness and accelerates early cell expansion.

Reduces Stress and Supports Survival:Stabilin? Protein reduces oxidative and mechanical stress, improving viability in the early culture phase.

Ready-to-use, Xeno-Free: Suitable for preclinical or research-grade BM-MSC expansion under defined, xeno-free conditions.

Components

Instructions for Use

1. Stock solution Preparation.

One kit is for 50ml cell culture medium.

The compounds are offered as powder in tubes. Please centrifuge before opening the cap to ensure the accuracy of the dosage.

Please carry out dissolution and packaging operations on a clean bench.

Spray the medium bottle and supplement tube with 70% ethanol and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior  threads  with  fingers. 

Reconstitute NaC20140103-A, B, C in sterile 0.9% NaCl solution. Aliquot into appropriate volumes of storage solution. The recommend volume is 1 mL each. Aliquot into appropriate volumes of storage solution. When stored at -20℃, the stock solution is stable for 6 month. When stored at 4 ℃, the stock solution is stable for 1 week.

Reconstitute NaC20140103-D in sterile 0.9% NaCl solution. The recommend volume is 5 mL. Aliquot into appropriate volumes of storage solution. When stored at 2-8 ℃, -20℃, the stock solution is stable for 24 month.

2. Reference Protocol

Step 1: Culture medium Preparation

a.  Thaw stock solutions of Lolina? Human BM-MSC stem-Prom? supplement kit 2.

b.  Dilute the stock solutions into regular culture medium without L-Glutamine to get complete culture medium.  

Step 2: Cell culture

c.  Rapidly thaw frozen BM-MSC vials in a 37°C water bath.

d.  Transfer cells to a sterile conical tube with pre-warmed medium.

e.  Centrifuge at 300g for 5 minutes to pellet cells.

f.  Resuspend the cell pellet in complete culture medium plate into culture dishes/flasks.

g.  Change the medium every 2-3 days.

Note

Cell Density: Avoid over-confluence to maintain MSC multipotency.

Medium Preparation: Always prepare fresh medium, even though SafeGluta? enhances stability.

Contamination Control: Use aseptic techniques to prevent contamination.

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